Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2B6

Concentration

0.15 mg/ml

View all SOX17 Antibodies »

Product Summary for SOX17 Antibody (OTI2B6)

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 177-414 of human SOX17

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens SRY (sex determining region Y)-box 17 (SOX17).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOX17 Antibody (OTI2B6)

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996]

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996]

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SOX17 Antibody (OTI2B6) [NBP1-47996]

Immunocytochemistry/ Immunofluorescence: SOX17 Antibody (OTI2B6) [NBP1-47996]

Immunocytochemistry/Immunofluorescence: SOX17 Antibody (OTI2B6) [NBP1-47996] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17.
Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996]

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996]

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996] - Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
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Applications for SOX17 Antibody (OTI2B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.15 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOX17

SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins

Long Name

Transcription Factor SOX17

Alternate Names

FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3

Entrez Gene IDs

64321 (Human)

Gene Symbol

SOX17

UniProt

Q9H6I2

Additional SOX17 Products

Product Documents for SOX17 Antibody (OTI2B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SOX17 Antibody (OTI2B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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