SOX17 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24568
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Predicted:
Primate (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 70-120 of human SOX17 was used as the immunogen for the antibody.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOX17 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SOX17 Antibody - BSA Free [NBP2-24568]
Immunocytochemistry/Immunofluorescence: SOX17 Antibody [NBP2-24568] - MCF7 cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Sox17 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.Western Blot: SOX17 AntibodyBSA Free [NBP2-24568]
Western Blot: SOX17 Antibody [NBP2-24568] - Analysis of SOX17 in mouse embryo brain lysate in the (1) absence and (2) presence of immunizing peptide using SOX17 antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: SOX17 Antibody - BSA Free [NBP2-24568]
Immunohistochemistry-Paraffin: SOX17 Antibody [NBP2-24568] - Human uterus (top) and testis (bottom) stained with SOX17 antibody at 10 ug/ml.Applications for SOX17 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOX17
Long Name
Transcription Factor SOX17
Alternate Names
FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3
Gene Symbol
SOX17
UniProt
Additional SOX17 Products
Product Documents for SOX17 Antibody - BSA Free
Product Specific Notices for SOX17 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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