SOX2 Antibody (2E1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26305
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2E1
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human SOX2 [UniProt P48431]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOX2 Antibody (2E1) - BSA Free
Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] -
Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] - Image of SOX2 Antibody (2E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SOX2 Antibody (2E1) [NBP3-26305] -
Western Blot: SOX2 Antibody (2E1) [NBP3-26305] - Positive Western Blot detected in: U-251 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate.All lanes: SOX2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 35 kDa
Observed band size: 40 kDa
Applications for SOX2 Antibody (2E1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SOX2
Long Name
Transcription Factor SOX2
Alternate Names
ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2
Gene Symbol
SOX2
Additional SOX2 Products
Product Documents for SOX2 Antibody (2E1) - BSA Free
Product Specific Notices for SOX2 Antibody (2E1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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