SOX2 Antibody (4G8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29623

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Chicken (93%), Mouse (96%), Primate (99%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4G8

Format

BSA Free

Concentration

1.0 mg/ml

View all SOX2 Antibodies »

Product Specifications

Immunogen

Full length recombinant human SOX2 (NCBI NP_003097).

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (96%), Chicken (93%), Sheep (99%) and Monkey/Primate species (99%), Zebrafish (87%), Xenopus tropicalis (87%), Xenopus laevis (88%).

Localization

Nucleus, Cytoplasm

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SOX2 Antibody (4G8) - BSA Free

Western Blot: SOX2 Antibody (4G8)BSA Free [NBP2-29623]

Western Blot: SOX2 Antibody (4G8)BSA Free [NBP2-29623]

Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.
Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - BSA Free [NBP2-29623]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - BSA Free [NBP2-29623]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].
Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - BSA Free [NBP2-29623]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) - BSA Free [NBP2-29623]

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].
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Applications for SOX2 Antibody (4G8) - BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX2

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Entrez Gene IDs

6657 (Human); 20674 (Mouse)

Gene Symbol

SOX2

OMIM

206900 (Human)

UniProt

P48431

Additional SOX2 Products

Product Documents for SOX2 Antibody (4G8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX2 Antibody (4G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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