Skip to main content

SOX2 Antibody (CL4716) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44099

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4716

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P48431, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX2 Antibody (CL4716) - Azide and BSA Free

SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human cerebellum shows weak to moderate nuclear positivity in Bergmann glia cells.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human liver shows no positivity in hepatocytes as expected.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human skin shows moderate to strong nuclear positivity in basal cell layer.

Applications for SOX2 Antibody (CL4716) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX2

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

Additional SOX2 Products

Product Documents for SOX2 Antibody (CL4716) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOX2 Antibody (CL4716) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...