SOX4 Antibody (PCRP-SOX4-1D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13777

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PCRP-SOX4-1D6

Concentration

0.2 mg/ml

View all SOX4 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa45-130) of human SOX4 protein (Uniprot: Q06945)

Reactivity Notes

Predicted to react in Xenopus and Chicken.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14180)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SOX4 Antibody (PCRP-SOX4-1D6)

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Immunocytochemistry/Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777]

Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
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Applications for SOX4 Antibody (PCRP-SOX4-1D6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SOX4

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Alternate Names

ecotropic viral integration site 16, EVI16, SRY (sex determining region Y)-box 4, SRY-related HMG-box gene 4, transcription factor SOX-4

Gene Symbol

SOX4

UniProt

Q06945 (Human)

Additional SOX4 Products

Product Documents for SOX4 Antibody (PCRP-SOX4-1D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX4 Antibody (PCRP-SOX4-1D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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