SOX9 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24659
![Simple Western: SOX9 Antibody [NBP2-24659] Simple Western: SOX9 Antibody [NBP2-24659]](https://resources.bio-techne.com/images/products/SOX9-Antibody-Simple-Western-NBP2-24659-img0004.jpg)
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Feline, Goat, Primate, Sheep
Cited:
Human, Mouse, Ovine
Predicted:
Bovine (93%), Chimpanzee (93%), Equine (100%), Monkey (100%), Opossum (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 225-275 of human SOX9 was used as the immunogen for this antibody.
Reactivity Notes
Sheep reactivity reported in scientific literature (PMID: 29624608). The amino acid sequence used as immunogen for the ZBTB2 antibody is 100% homologous in monkey, opossum and horse, and 93% homologous in cow and chimpanzee.
Marker
Sertoli Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOX9 Antibody - BSA Free
Simple Western: SOX9 Antibody [NBP2-24659]
Simple Western: SOX9 Antibody [NBP2-24659] - Simple Western lane view shows a specific band for SOX9 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunohistochemistry: SOX9 Antibody [NBP2-24659]
Immunohistochemistry: SOX9 Antibody [NBP2-24659] - Osteo-chondroprogenitor cells in the zone adjacent to the titanium plate surface are positive for Sox9 (arrows). Picture on the left represent Group 0.1, pictures on the right represent Group 0.6. 100-fold (c-d) magnification. PLoS One. 2018 Apr 6;13(4):e0195466. doi: 10.1371/journal.pone.0195466.Applications for SOX9 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/ml
Simple Western
1:50
Western Blot
2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 51 kDa.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 51 kDa.
Reviewed Applications
Read 1 review rated 3 using NBP2-24659 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOX9
Long Name
Transcription Factor SOX9
Alternate Names
campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9
Gene Symbol
SOX9
UniProt
Additional SOX9 Products
Product Documents for SOX9 Antibody - BSA Free
Product Specific Notices for SOX9 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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