Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1.7

Concentration

1.0 mg/ml

View all SP-D Antibodies »

Product Specifications

Immunogen

Purified porcine surfactant protein D

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26092997).

Specificity

This antibody detects porcine surfactant protein D (pSP-D), a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SP-D Antibody (1.7)

Immunohistochemistry-Paraffin: SP-D Antibody (1.7) - BSA Free [NBP1-41050]

Immunohistochemistry-Paraffin: SP-D Antibody (1.7) - BSA Free [NBP1-41050]

Immunohistochemistry-Paraffin: SP-D Antibody (1.7) [NBP1-41050] - Staining of frozen pig lung with Mouse anti pig Surfactant Protein D

Applications for SP-D Antibody (1.7)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; TRIS/EDTA buffer pH8.0 is recommended for this purpose.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP-D

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).

Long Name

Surfactant Pulmonary Associated Protein D

Alternate Names

Collectin 7, PSP-D, SFTP4, SFTPD, SPD

Entrez Gene IDs

397198 (Porcine)

Gene Symbol

SFTPD

UniProt

Q9N1X4 (Porcine)

Additional SP-D Products

Product Documents for SP-D Antibody (1.7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SP-D Antibody (1.7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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