SP1 Antibody (1A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006667-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Specifications

Immunogen

SP1 (NP_612482, 89 a.a. ~ 198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

SP1 (1A5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SP1 Antibody (1A5)

Western Blot: SP1 Antibody (1A5) [H00006667-M02]

Western Blot: SP1 Antibody (1A5) [H00006667-M02]

Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5 Analysis of SP1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02]

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02]

Immunocytochemistry/Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02]

Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02]

Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
View 3 more images

Applications for SP1 Antibody (1A5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Entrez Gene IDs

6667 (Human); 20683 (Mouse); 24790 (Rat)

Gene Symbol

SP1

OMIM

189906 (Human)

UniProt

NP_612482 (Human)

Additional SP1 Products

Product Documents for SP1 Antibody (1A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SP1 Antibody (1A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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