SP1 Antibody (4C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006667-M03

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Specifications

Immunogen

SP1 (NP_612482, 89 a.a. ~ 198 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQ

Specificity

SP1 (4C8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SP1 Antibody (4C8)

Western Blot: SP1 Antibody (4C8) [H00006667-M03]

Western Blot: SP1 Antibody (4C8) [H00006667-M03] - Analysis of SP1 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (4C8) [H00006667-M03]

Immunocytochemistry/Immunofluorescence: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 20 ug/ml

Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03]

Immunohistochemistry-Paraffin: SP1 Antibody (4C8) [H00006667-M03] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml

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Applications for SP1 Antibody (4C8)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Entrez Gene IDs

6667 (Human)

Gene Symbol

SP1

OMIM

189906 (Human)

UniProt

NP_612482 (Human)

Additional SP1 Products

Product Documents for SP1 Antibody (4C8)

Product Datasheets

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COA

SDS

Product Specific Notices for SP1 Antibody (4C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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