SP1 Antibody (5I3A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15837

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15837-100ul
NBP3-15837-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5I3A8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Summary for SP1 Antibody (5I3A8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047). TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP1 Antibody (5I3A8)

Western Blot: SP1 Antibody (5I3A8) [NBP3-15837]

Western Blot: SP1 Antibody (5I3A8) [NBP3-15837]

Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837]

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837]

Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837]

Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837]

Immunohistochemistry-Paraffin: SP1 Antibody (5I3A8) [NBP3-15837] - Immunohistochemistry of paraffin-embedded mouse kidney using SP1 antibody (NBP3-15837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for SP1 Antibody (5I3A8)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:50 - 1:200

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody (5I3A8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SP1 Antibody (5I3A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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