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SP1 Antibody (GT2574)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13611

Novus Biologicals, part of Bio-Techne
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NBP3-13611

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (96%), Rat (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT2574

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SP1 Antibody (GT2574)

SP1 Antibody (GT2574)

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (GT2574) [NBP3-13611] -

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (GT2574) [NBP3-13611] - SP1 antibody [GT2574] detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody [GT2574] (NBP3-13611) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Scale bar= 10um.
Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611]

Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611]

Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611] - SP1 antibody [GT2574] detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SP1 stained by SP1 antibody [GT2574] (NBP3-13611) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SP1 Antibody (GT2574)

Western Blot: SP1 Antibody (GT2574) [NBP3-13611] -

Western Blot: SP1 Antibody (GT2574) [NBP3-13611] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT2574] (NBP3-13611) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for SP1 Antibody (GT2574)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody (GT2574)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SP1 Antibody (GT2574)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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