SP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85812

Novus Biologicals, part of Bio-Techne
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NBP1-85812
NBP1-85812-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP1 Antibodies »

Product Summary for SP1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP1 Antibody

Western Blot: SP1 Antibody [NBP1-85812]

Western Blot: SP1 Antibody [NBP1-85812]

Western Blot: SP1 Antibody [NBP1-85812] - Analysis using Anti-SP1 antibody NBP1-85812 (A) shows similar pattern to independent antibody NBP1-89458 (B).
Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP1-85812]

Immunocytochemistry/ Immunofluorescence: SP1 Antibody [NBP1-85812]

Immunocytochemistry/Immunofluorescence: SP1 Antibody [NBP1-85812] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-85812]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-85812]

Immunohistochemistry-Paraffin: SP1 Antibody [NBP1-85812] - Staining of human small intestine shows nuclear positivity in glandular cells.

Applications for SP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Alternate Names

Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1

Gene Symbol

SP1

Additional SP1 Products

Product Documents for SP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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