SP100 Antibody (PCRP-SP100-1B9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13780
![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]](https://resources.bio-techne.com/images/products/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0003.jpg "Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]")
![Protein Array: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]](https://resources.bio-techne.com/images/products/SP100-Antibody-PCRP-SP100-1B9-Protein-Array-NBP3-13780-img0006.jpg "Protein Array: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]")
![Immunocytochemistry/ Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]](https://resources.bio-techne.com/images/products/SP100-Antibody-PCRP-SP100-1B9-Immunocytochemistry-Immunofluorescence-NBP3-13780-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-SP100-1B9
Concentration
0.2 mg/ml
Product Summary for SP100 Antibody (PCRP-SP100-1B9)
Immunogen
Recombinant full-length human SP100 protein (Uniprot: P23497)
Localization
Nucleus, PML body
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
100.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14183)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for SP100 Antibody (PCRP-SP100-1B9)
Immunocytochemistry/ Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]
Immunocytochemistry/Immunofluorescence: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.![Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]](https://resources.bio-techne.com/images/products/SP100-Antibody-PCRP-SP100-1B9-Immunohistochemistry-Paraffin-NBP3-13780-img0004.jpg "Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]")
Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]
Immunohistochemistry-Paraffin: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using SP100 antibody (PCRP-SP100-1B9) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]](https://resources.bio-techne.com/images/products/SP100-Antibody-PCRP-SP100-1B9-Flow-Cytometry-NBP3-13780-img0001.jpg "Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]")
Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780]
Flow Cytometry: SP100 Antibody (PCRP-SP100-1B9) [NBP3-13780] - Flow cytometric analysis of PFA-fixed HeLa cells. SP100 antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Applications for SP100 Antibody (PCRP-SP100-1B9)
Application
Recommended Usage
Flow Cytometry
0.5-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SP100
Alternate Names
DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa
Gene Symbol
SP100
UniProt
Additional SP100 Products
Product Documents for SP100 Antibody (PCRP-SP100-1B9)
Product Specific Notices for SP100 Antibody (PCRP-SP100-1B9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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