SP100 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89457
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SP100 Antibody
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457]
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-89457 (A) shows similar protein distribution across tissues to independent antibody NBP1-86060 (B).Immunocytochemistry/ Immunofluorescence: SP100 Antibody [NBP1-89457]
Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP1-89457] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457]
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-89457] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.Applications for SP100 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SP100
Alternate Names
DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa
Gene Symbol
SP100
Additional SP100 Products
Product Documents for SP100 Antibody
Product Specific Notices for SP100 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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