SP17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85409

Novus Biologicals, part of Bio-Techne
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NBP1-85409
NBP1-85409-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP17 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP17 Antibody

Western Blot: SP17 Antibody [NBP1-85409]

Western Blot: SP17 Antibody [NBP1-85409]

Western Blot: SP17 Antibody [NBP1-85409] - Analysis in control (vector only transfected HEK293T lysate) and SPA17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SP17 Antibody [NBP1-85409]

Immunocytochemistry/ Immunofluorescence: SP17 Antibody [NBP1-85409]

Immunocytochemistry/Immunofluorescence: SP17 Antibody [NBP1-85409] - Staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409]

Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409]

Immunohistochemistry-Paraffin: SP17 Antibody [NBP1-85409] - Staining in human testis and endometrium tissues using anti-SPA17 antibody. Corresponding SPA17 RNA-seq data are presented for the same tissues.
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Applications for SP17 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP17

SP17 encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Alternate Names

Cancer/testis antigen 22, CT22SP17-1, Sp17-1, SP17sperm surface protein Sp17, sperm autoantigenic protein 17Sperm protein 17

Gene Symbol

SPA17

Additional SP17 Products

Product Documents for SP17 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SP17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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