SPAM1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93736

Novus Biologicals, part of Bio-Techne
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NBP2-93736-0.02ml
NBP2-93736-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPAM1 Antibodies »

Product Summary for SPAM1 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). PYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAM1 Antibody - Azide and BSA Free

Western Blot: SPAM1 AntibodyAzide and BSA Free [NBP2-93736]

Western Blot: SPAM1 AntibodyAzide and BSA Free [NBP2-93736]

Western Blot: SPAM1 Antibody [NBP2-93736] - Analysis of extracts of various cell lines, using SPAM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/Immunofluorescence: SPAM1 Antibody [NBP2-93736] - Analysis of human testis cells using SPAM1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunohistochemistry-Paraffin: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP2-93736] - Rat testis using SPAM1 Rabbit pAb at dilution of 1:100 (40x lens).
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Applications for SPAM1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Long Name

Sperm Adhesion Molecule 1

Alternate Names

HYA1, HYAL3, HYAL5, PH20, SPAG15

Gene Symbol

SPAM1

Additional SPAM1 Products

Product Documents for SPAM1 Antibody - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for SPAM1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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