SPARC Antibody (001)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90612
Recombinant Monoclonal Antibody
![Western Blot: SPARC Antibody (001) [NBP2-90612]](https://resources.bio-techne.com/images/products/SPARC-Antibody-001-Western-Blot-NBP2-90612-img0003.jpg "Western Blot: SPARC Antibody (001) [NBP2-90612]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SPARC (Accession#: NP_033268.1; Met1-Ile302).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SPARC Antibody (001)
Western Blot: SPARC Antibody (001) [NBP2-90612]
Western Blot: SPARC Antibody (001) [NBP2-90612] - Anti-SPARC rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 40 kDa![Immunoprecipitation: SPARC Antibody (001) [NBP2-90612]](https://resources.bio-techne.com/images/products/SPARC-Antibody-001-Immunoprecipitation-NBP2-90612-img0004.jpg "Immunoprecipitation: SPARC Antibody (001) [NBP2-90612]")
Immunoprecipitation: SPARC Antibody (001) [NBP2-90612]
Immunoprecipitation: SPARC Antibody (001) [NBP2-90612] - Mouse SPARC was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 0.5 ul anti-Mouse SPARC rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse SPARC rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDaApplications for SPARC Antibody (001)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPARC
Long Name
Secreted Protein Acidic and Rich in Cysteine
Alternate Names
BM-40, Osteonectin
Gene Symbol
SPARC
Additional SPARC Products
Product Documents for SPARC Antibody (001)
Product Specific Notices for SPARC Antibody (001)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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