SPARC Antibody (PD00-57)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32974
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PD00-57
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide. (Uniprot: P09486)
Localization
Secreted, extracellular space, extracellular matrix, basement membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SPARC Antibody (PD00-57)
Immunohistochemistry: SPARC Antibody (PD00-57) [NBP3-32974]
Immunohistochemistry: SPARC Antibody (PD00-57) [NBP3-32974] - Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Rabbit anti-SPARC antibody (NBP3-32974) at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32974) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for SPARC Antibody (PD00-57)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPARC
Long Name
Secreted Protein Acidic and Rich in Cysteine
Alternate Names
BM-40, Osteonectin
Gene Symbol
SPARC
Additional SPARC Products
Product Documents for SPARC Antibody (PD00-57)
Product Specific Notices for SPARC Antibody (PD00-57)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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