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SPATA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38302

Novus Biologicals, part of Bio-Techne
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NBP2-38302
NBP2-38302-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGNEQLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPATA5 Antibody

Western Blot: SPATA5 Antibody [NBP2-38302]

Western Blot: SPATA5 Antibody [NBP2-38302]

Western Blot: SPATA5 Antibody [NBP2-38302] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: SPATA5 Antibody [NBP2-38302]

Immunocytochemistry/ Immunofluorescence: SPATA5 Antibody [NBP2-38302]

Immunocytochemistry/Immunofluorescence: SPATA5 Antibody [NBP2-38302] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SPATA5 Antibody [NBP2-38302]

Immunohistochemistry-Paraffin: SPATA5 Antibody [NBP2-38302]

Immunohistochemistry-Paraffin: SPATA5 Antibody [NBP2-38302] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Applications for SPATA5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPATA5

SPATA5 may be involved in morphological and functional mitochondrial transformations during spermatogenesis (Bysimilarity)

Alternate Names

AFG2ATPase family gene 2 homolog, ATPase family protein 2 homolog, SPAFspermatogenesis associated factor SPAF, spermatogenesis associated 5, Spermatogenesis-associated factor protein, spermatogenesis-associated protein 5

Gene Symbol

AFG2A

UniProt

Additional SPATA5 Products

Product Documents for SPATA5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPATA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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