SPATA9 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83493
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKKVKNIFQEEESIRQNREESENCRKAFSEPVLSEPMFAEGEIKAKPYRSLPEKPDISDYPKLLANKQSNNIQVLHSVFDQSAEMNEQI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SPATA9 Antibody
Western Blot: SPATA9 Antibody [NBP1-83493]
Western Blot: SPATA9 Antibody [NBP1-83493] - Analysis in control (vector only transfected HEK293T lysate) and SPATA9 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: SPATA9 Antibody [NBP1-83493]
Immunocytochemistry/Immunofluorescence: SPATA9 Antibody [NBP1-83493] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SPATA9 Antibody [NBP1-83493]
Immunohistochemistry-Paraffin: SPATA9 Antibody [NBP1-83493] - Staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.Applications for SPATA9 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SPATA9
Alternate Names
FLJ35906, NYD-SP16, spermatogenesis associated 9, spermatogenesis-associated protein 9, Testis development protein NYD-SP16
Gene Symbol
SPATA9
Additional SPATA9 Products
Product Documents for SPATA9 Antibody
Product Specific Notices for SPATA9 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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