SPC25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47263

Novus Biologicals, part of Bio-Techne
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NBP2-47263
NBP2-47263-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPC25 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPC25 Antibody

Western Blot: SPC25 Antibody [NBP2-47263]

Western Blot: SPC25 Antibody [NBP2-47263]

Western Blot: SPC25 Antibody [NBP2-47263] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: SPC25 Antibody [NBP2-47263]

Immunocytochemistry/ Immunofluorescence: SPC25 Antibody [NBP2-47263]

Immunocytochemistry/Immunofluorescence: SPC25 Antibody [NBP2-47263] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: SPC25 Antibody [NBP2-47263]

Immunohistochemistry-Paraffin: SPC25 Antibody [NBP2-47263]

Immunohistochemistry-Paraffin: SPC25 Antibody [NBP2-47263] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.

Applications for SPC25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPC25

SPC25 encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq]

Alternate Names

AD024, hSpc25, kinetochore protein Spc25, MGC22228,2600017H08Rik, SPBC25, SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae), spindle pole body component 25 homolog, spindle pole body component 25 homolog (S. cerevisiae)

Gene Symbol

SPC25

Additional SPC25 Products

Product Documents for SPC25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPC25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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