Spectrin alpha 1 Antibody (SR1450)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21928

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21928-100ul
NBP3-21928-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1450

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Spectrin alpha 1 Antibodies »

Product Summary for Spectrin alpha 1 Antibody (SR1450)

Immunogen

A synthesized peptide derived from human Spectrin alpha 1 (Uniprot #: P02549)

Specificity

Detects endogenous levels of total Spectrin alpha 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Spectrin alpha 1 Antibody (SR1450)

Western Blot: Spectrin alpha 1 Antibody (SR1450) [NBP3-21928] -

Western Blot: Spectrin alpha 1 Antibody (SR1450) [NBP3-21928] - Western blot analysis using NBP3-21928 in K562 cell lysate.

Applications for Spectrin alpha 1 Antibody (SR1450)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spectrin alpha 1

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Alternate Names

alpha-I spectrin, EL2, erythrocyte, HS3, spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Gene Symbol

SPTA1

Additional Spectrin alpha 1 Products

Product Documents for Spectrin alpha 1 Antibody (SR1450)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin alpha 1 Antibody (SR1450)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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