Spectrin alpha 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87953

Novus Biologicals, part of Bio-Techne
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NBP1-87953
NBP1-87953-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Spectrin alpha 1 Antibodies »

Product Summary for Spectrin alpha 1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVEL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spectrin alpha 1 Antibody

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953] - Staining of human placenta shows moderate membranous positivity in erythrocytes.
Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953] - Staining of human bone marrow shows moderate membranous positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody [NBP1-87953] - Staining of human endometrium shows no positivity in glandular cells as expected.
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Applications for Spectrin alpha 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spectrin alpha 1

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Alternate Names

alpha-I spectrin, EL2, erythrocyte, HS3, spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Gene Symbol

SPTA1

Additional Spectrin alpha 1 Products

Product Documents for Spectrin alpha 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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