Spectrin beta 1 Antibody (RG/26) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08440

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # RG/26

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Spectrin beta 1 Antibodies »

Product Summary for Spectrin beta 1 Antibody (RG/26) - Azide and BSA Free

Immunogen

Red cell membranes

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

246 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07292).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Spectrin beta 1 Antibody (RG/26) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Spectrin beta 1

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Alternate Names

beta-I spectrin, EL3, erythrocyte, HS2, HSPTB1, membrane cytoskeletal protein, spectrin, beta, erythrocytic, SPTB1

Gene Symbol

SPTB

Additional Spectrin beta 1 Products

Product Documents for Spectrin beta 1 Antibody (RG/26) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Spectrin beta 1 Antibody (RG/26) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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