Spectrin beta 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90350

Novus Biologicals, part of Bio-Techne
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NBP1-90350
NBP1-90350-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Spectrin beta 1 Antibodies »

Product Summary for Spectrin beta 1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spectrin beta 1 Antibody

Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody [NBP1-90350]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody [NBP1-90350]

Immunocytochemistry/Immunofluorescence: Spectrin beta 1 Antibody [NBP1-90350] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350]

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350]

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350]

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350]

Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody [NBP1-90350] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
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Applications for Spectrin beta 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spectrin beta 1

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Alternate Names

beta-I spectrin, EL3, erythrocyte, HS2, HSPTB1, membrane cytoskeletal protein, spectrin, beta, erythrocytic, SPTB1

Gene Symbol

SPTB

Additional Spectrin beta 1 Products

Product Documents for Spectrin beta 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin beta 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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