Spectrin beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86088

Novus Biologicals, part of Bio-Techne
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NBP1-86088
NBP1-86088-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Spectrin beta 2 Antibodies »

Product Summary for Spectrin beta 2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spectrin beta 2 Antibody

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Analysis in human lung and skeletal muscle tissues using NBP1-86088 antibody. Corresponding SPTBN1 RNA-seq data are presented for the same tissues.
Western Blot: Spectrin beta 2 Antibody [NBP1-86088]

Western Blot: Spectrin beta 2 Antibody [NBP1-86088]

Western Blot: Spectrin beta 2 Antibody [NBP1-86088] - Analysis in human cell line WM-115.
Immunocytochemistry/ Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088]

Immunocytochemistry/Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
View 5 more images

Applications for Spectrin beta 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spectrin beta 2

Spectrin Beta 2 is a major constituent of the erythrocyte skeleton. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol.

Alternate Names

beta-fodrin, beta-G spectrin, Beta-II spectrin, beta-spectrin 2, beta-spectrin II, betaSpII, ELF, embryonic liver beta-fodrin, Fodrin beta chain, spectrin beta chain, brain 1, spectrin, beta, non-erythrocytic 1, Spectrin, non-erythroid beta chain 1, SPTB2

Gene Symbol

SPTBN1

Additional Spectrin beta 2 Products

Product Documents for Spectrin beta 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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