SPFH2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1884
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Chinese Hamster (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EDEPLETATKEN corresponding to C-Terminus according to NP_009106.1.
Reactivity Notes
Chinese Hamster reactivity reported in scientific literature (PMID: 21343306).
Specificity
This antibody is expected to recognise isoform 1 (NP_009106) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SPFH2 Antibody
![Western Blot: SPFH2 Antibody [NB100-1884]](https://resources.bio-techne.com/images/products/SPFH2-Antibody-Western-Blot-NB100-1884-img0002.jpg "Western Blot: SPFH2 Antibody [NB100-1884]")
Western Blot: SPFH2 Antibody [NB100-1884]
Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.![Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884]](https://resources.bio-techne.com/images/products/SPFH2-Antibody-Immunohistochemistry-Paraffin-NB100-1884-img0003.jpg "Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884]")
Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884]
Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for SPFH2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
Detection limit 1:2000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 43 kDa band observed in human breast lysates and in lysates of human breast cancer (PMID: 17502376).
IHC: Paraffin embedded Human Breast.
IHC: Paraffin embedded Human Breast.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPFH2
Alternate Names
C8orf2SPFH domain-containing protein 2, Endoplasmic reticulum lipid raft-associated protein 2, ER lipid raft associated 2, Erlin-2, MGC87072, NET32, SPFH domain family, member 2, SPFH2chromosome 8 open reading frame 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
Gene Symbol
ERLIN2
UniProt
Additional SPFH2 Products
Product Documents for SPFH2 Antibody
Product Specific Notices for SPFH2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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