SPG7 Antibody (OTI1C1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74327
![Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0001.jpg "Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]")
![Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0003.jpg "Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1C1
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
88.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SPG7 Antibody (OTI1C1) - Azide and BSA Free
Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]
Western Blot: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Human tissue.![Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74327-img0007.jpg "Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]")
Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]
Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.![Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74327-img0005.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]")
Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]
Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Western-Blot-NBP2-74327-img0002.jpg "Western Blot: SPG7 Antibody (OTI1C1)Azide and BSA Free [NBP2-74327]")
![Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74327-img0006.jpg "Immunocytochemistry/ Immunofluorescence: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]")
![Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]](https://resources.bio-techne.com/images/products/SPG7-Antibody-OTI1C1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74327-img0004.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) - Azide and BSA Free [NBP2-74327]")
Applications for SPG7 Antibody (OTI1C1) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SPG7
Alternate Names
CARMGC126331, cell matrix adhesion regulator, CMARMGC126332, EC 3.4.24, EC 3.4.24.-, FLJ37308, paraplegin, PGNcell adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), Spastic paraplegia 7 protein, SPG5C
Gene Symbol
SPG7
Additional SPG7 Products
Product Documents for SPG7 Antibody (OTI1C1) - Azide and BSA Free
Product Specific Notices for SPG7 Antibody (OTI1C1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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