Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SPG7 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment correspongding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPG7 Antibody (OTI1G4) - Azide and BSA Free

Western Blot: SPG7 Antibody (OTI1G4)Azide and BSA Free [NBP2-74323]

Western Blot: SPG7 Antibody (OTI1G4)Azide and BSA Free [NBP2-74323]

Western Blot: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human SPG7(NP_003110) gene (amino acids 300-573) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted w
Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323]

Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323]

Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323]

Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323]

Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for SPG7 Antibody (OTI1G4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPG7

SPG7 - spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

Alternate Names

CARMGC126331, cell matrix adhesion regulator, CMARMGC126332, EC 3.4.24, EC 3.4.24.-, FLJ37308, paraplegin, PGNcell adhesion regulator, spastic paraplegia 7 (pure and complicated autosomal recessive), Spastic paraplegia 7 protein, SPG5C

Gene Symbol

SPG7

Additional SPG7 Products

Product Documents for SPG7 Antibody (OTI1G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPG7 Antibody (OTI1G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.