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SPINK1 Antibody (PD00-93)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32975

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32975

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-93

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full length protein corresponding to Human SPINK1 aa 1 to the C-terminus. (Uniprot: P00995)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPINK1 Antibody (PD00-93)

SPINK1 Antibody (PD00-93)

Immunohistochemistry: SPINK1 Antibody (PD00-93) [NBP3-32975]

Immunohistochemistry: SPINK1 Antibody (PD00-93) [NBP3-32975] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-SPINK1 antibody (NBP3-32975) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32975) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for SPINK1 Antibody (PD00-93)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPINK1

SPINK1 (Serine protease inhibitor, Kazal type I) is a trypsin inhibitor which is secreted from pancreatic acinar cells into pancreatic juice. It prevents the trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct.

Long Name

Serine Peptidase Inhibitor, Kazal Type 1

Alternate Names

PCTT, PSTI, Spink3, TATI

Gene Symbol

SPINK1

Additional SPINK1 Products

Product Documents for SPINK1 Antibody (PD00-93)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPINK1 Antibody (PD00-93)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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