Splicing Factor 1 Antibody (2E12)

Name: Splicing Factor 1 Antibody (2E12)
Brand: Novus Biologicals
SKU: H00007536-M01

Novus Biologicals, part of Bio-Techne | Catalog # H00007536-M01

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERH

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

SF1 - splicing factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Splicing Factor 1 Antibody (2E12)

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in HeLa.

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in NIH/3T3.

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01A), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

Immunoprecipitation: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.

ELISA: Splicing Factor 1 Antibody (2E12) [H00007536-M01]

ELISA: Splicing Factor 1 Antibody (2E12.) [H00007536-M01] - Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.

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