Splicing Factor 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86835
![Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]](https://resources.bio-techne.com/images/products/Splicing-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86835-img0008.jpg "Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLI
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23435261), Mouse reactivity reported in scientific literature (PMID: 25586139).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Splicing Factor 1 Antibody
![Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835]](https://resources.bio-techne.com/images/products/Splicing-Factor-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86835-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835]")
Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835]
Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]](https://resources.bio-techne.com/images/products/Splicing-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86835-img0011.jpg "Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]")
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]](https://resources.bio-techne.com/images/products/Splicing-Factor-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86835-img0006.jpg "Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]")
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.Applications for Splicing Factor 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 25586139).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Splicing Factor 1
Alternate Names
Mammalian branch point-binding protein, mBBP, splicing factor 1, Transcription factor ZFM1, ZFM1BBP, Zinc finger gene in MEN1 locus, Zinc finger protein 162D11S636, ZNF162MBBP
Gene Symbol
SF1
Additional Splicing Factor 1 Products
Product Documents for Splicing Factor 1 Antibody
Product Specific Notices for Splicing Factor 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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