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Splicing Factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86835

Novus Biologicals, part of Bio-Techne
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NBP1-86835
NBP1-86835-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLI

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261), Mouse reactivity reported in scientific literature (PMID: 25586139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Splicing Factor 1 Antibody

Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835]

Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835]

Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835]

Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody [NBP1-86835] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Applications for Splicing Factor 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 25586139).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Splicing Factor 1

Alternate Names

Mammalian branch point-binding protein, mBBP, splicing factor 1, Transcription factor ZFM1, ZFM1BBP, Zinc finger gene in MEN1 locus, Zinc finger protein 162D11S636, ZNF162MBBP

Gene Symbol

SF1

Additional Splicing Factor 1 Products

Product Documents for Splicing Factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Splicing Factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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