SPR Antibody (4F2)
Novus Biologicals, part of Bio-Techne | Catalog # H00006697-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4F2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SPR (NP_003115, 164 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYD
Specificity
SPR - sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (4F2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for SPR Antibody (4F2)
![Western Blot: SPR Antibody (4F2) [H00006697-M01]](https://resources.bio-techne.com/images/products/SPR-Antibody-4F2-Western-Blot-H00006697-M01-img0003.jpg "Western Blot: SPR Antibody (4F2) [H00006697-M01]")
Western Blot: SPR Antibody (4F2) [H00006697-M01]
Western Blot: SPR Antibody (4F2) [H00006697-M01] - Analysis of SPR expression in transfected 293T cell line by SPR monoclonal antibody (M01), clone 4F2.Lane 1: SPR transfected lysate(28 KDa).Lane 2: Non-transfected lysate.![Immunoprecipitation: SPR Antibody (4F2) [H00006697-M01]](https://resources.bio-techne.com/images/products/SPR-Antibody-4F2-Immunoprecipitation-H00006697-M01-img0001.jpg "Immunoprecipitation: SPR Antibody (4F2) [H00006697-M01]")
Immunoprecipitation: SPR Antibody (4F2) [H00006697-M01]
Immunoprecipitation: SPR Antibody (4F2) [H00006697-M01] - Analysis of SPR transfected lysate using anti-SPR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SPR MaxPab rabbit polyclonal antibody.
Sandwich ELISA: SPR Antibody (4F2) [H00006697-M01] - Detection limit for recombinant GST tagged SPR is 0.3 ng/ml as a capture antibody.
Applications for SPR Antibody (4F2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SPR
Long Name
Sepiapterin Reductase
Alternate Names
EC 1.1.1.153, Gm10328, SDR38C1
Entrez Gene IDs
6697 (Human)
Gene Symbol
SPR
UniProt
Additional SPR Products
Product Documents for SPR Antibody (4F2)
Product Specific Notices for SPR Antibody (4F2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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