Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SPRED2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2. The immunogen is located within amino acids 140 - 190 of Spred2. Amino Acid Squence: SNSSQKREQPTRT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Specificity

This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPRED2 Antibody - BSA Free

Western Blot: SPRED2 AntibodyBSA Free [NBP1-76342]

Western Blot: SPRED2 AntibodyBSA Free [NBP1-76342]

Western Blot: SPRED2 Antibody [NBP1-76342] - Human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 ug/mL.

Applications for SPRED2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRED2

Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist. This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.

Long Name

Sprouty-related, EVH1 Domain Containing 2

Alternate Names

FLJ31917, MGC163164, Spred-2related sequence, sprouty-related, EVH1 domain containing 2, sprouty-related, EVH1 domain-containing protein 2

Gene Symbol

SPRED2

UniProt

NP_861449 (Human)

Additional SPRED2 Products

Product Documents for SPRED2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPRED2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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