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SPRED2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20478

Novus Biologicals, part of Bio-Techne
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NBP2-20478

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SPRED2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPRED2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque.

Localization

Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential); Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SPRED2 Antibody

Western Blot: SPRED2 Antibody [NBP2-20478]

Western Blot: SPRED2 Antibody [NBP2-20478]

Western Blot: SPRED2 Antibody [NBP2-20478] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SPRED2 Antibody [NBP2-20478]

Immunohistochemistry-Paraffin: SPRED2 Antibody [NBP2-20478]

Immunohistochemistry-Paraffin: SPRED2 Antibody [NBP2-20478] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Applications for SPRED2 Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRED2

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM]

Long Name

Sprouty-related, EVH1 Domain Containing 2

Alternate Names

FLJ31917, MGC163164, Spred-2related sequence, sprouty-related, EVH1 domain containing 2, sprouty-related, EVH1 domain-containing protein 2

Gene Symbol

SPRED2

UniProt

Additional SPRED2 Products

Product Documents for SPRED2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPRED2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.