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SPRR2F Antibody (5A9)

Novus Biologicals, part of Bio-Techne | Catalog # H00006705-M03

Novus Biologicals, part of Bio-Techne
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H00006705-M03

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5A9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SPRR2F (NP_001014450.1, 1 a.a. ~ 72 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSYQQQQCKQPCQPPPVCPAPKCPEPCPPPKCPEPCPPSKCPQSCPPQQCQQKCPPVTPSPPCQPKCPPKSK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SPRR2F Antibody (5A9)

Western Blot: SPRR2F Antibody (5A9) [H00006705-M03]

Western Blot: SPRR2F Antibody (5A9) [H00006705-M03]

Western Blot: SPRR2F Antibody (5A9) [H00006705-M03] - Analysis of SPRR2F expression in transfected 293T cell line by SPRR2F monoclonal antibody (M03), clone 5A9. Lane 1: SPRR2F transfected lysatE (7.8 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: SPRR2F Antibody (5A9) [H00006705-M03] - Detection limit for recombinant GST tagged SPRR2F is 0.1 ng/ml as a capture antibody.

Applications for SPRR2F Antibody (5A9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRR2F

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane

Alternate Names

small proline-rich protein 2F, SPR-2F

Gene Symbol

SPRR2F

Additional SPRR2F Products

Product Documents for SPRR2F Antibody (5A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPRR2F Antibody (5A9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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