SPRY2 Antibody (1E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00010253-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPRY2 Antibodies »

Product Specifications

Immunogen

SPRY2 (AAH15745.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT

Localization

Cytoplasm. Cell membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).

Specificity

SPRY2 - sprouty homolog 2 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SPRY2 Antibody (1E10)

Western Blot: SPRY2 Antibody (1E10) [H00010253-M01]

Western Blot: SPRY2 Antibody (1E10) [H00010253-M01]

Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.
Immunocytochemistry/ Immunofluorescence: SPRY2 Antibody (1E10) [H00010253-M01]

Immunocytochemistry/ Immunofluorescence: SPRY2 Antibody (1E10) [H00010253-M01]

Immunocytochemistry/Immunofluorescence: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on HeLa cell. Antibody concentration 25 ug/ml.
Immunohistochemistry-Paraffin: SPRY2 Antibody (1E10) [H00010253-M01]

Immunohistochemistry-Paraffin: SPRY2 Antibody (1E10) [H00010253-M01]

Immunohistochemistry-Paraffin: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
View 2 more images

Applications for SPRY2 Antibody (1E10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRY2

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Long Name

Sprouty Homolog 2

Alternate Names

sprouty 2

Entrez Gene IDs

10253 (Human)

Gene Symbol

SPRY2

OMIM

602466 (Human)

UniProt

AAH15745 (Human)

Additional SPRY2 Products

Product Documents for SPRY2 Antibody (1E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPRY2 Antibody (1E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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