SPRY4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56160

Novus Biologicals, part of Bio-Techne
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NBP2-56160
NBP2-56160-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPRY4 Antibodies »

Product Summary for SPRY4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LEACFSVQSRTSSPMEPPIPQSAPLTPNSVMVQPLLDSRMSHSRLQHPLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPRY4 Antibody

Western Blot: SPRY4 Antibody [NBP2-56160]

Western Blot: SPRY4 Antibody [NBP2-56160]

Western Blot: SPRY4 Antibody [NBP2-56160] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: SPRY4 Antibody [NBP2-56160]

Immunocytochemistry/ Immunofluorescence: SPRY4 Antibody [NBP2-56160]

Immunocytochemistry/Immunofluorescence: SPRY4 Antibody [NBP2-56160] - Staining of human cell line PC-3 shows localization to cytosol & the Golgi apparatus.

Applications for SPRY4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRY4

Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.

Long Name

Sprouty Homolog 4

Alternate Names

protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry-4

Gene Symbol

SPRY4

Additional SPRY4 Products

Product Documents for SPRY4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPRY4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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