Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SPRYD4 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4. The immunogen is located within amino acids 70 - 120 of SPRYD4. Amino Acid Squence: VTSGRHYWEVTVKRSQQF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%), Porcine (89%)

Specificity

SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPRYD4 Antibody - BSA Free

Western Blot: SPRYD4 AntibodyBSA Free [NBP1-77100]

Western Blot: SPRYD4 AntibodyBSA Free [NBP1-77100]

Western Blot: SPRYD4 Antibody [NBP1-77100] - Analysis in fetal human liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide
SPRYD4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SPRYD4 Antibody - BSA Free [NBP1-77100] -

Immunocytochemistry/ Immunofluorescence: SPRYD4 Antibody - BSA Free [NBP1-77100] - Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 u/mL.
SPRYD4 Antibody - BSA Free

Immunohistochemistry: SPRYD4 Antibody - BSA Free [NBP1-77100] -

Immunohistochemistry: SPRYD4 Antibody - BSA Free [NBP1-77100] - Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 u/mL.

Applications for SPRYD4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPRYD4

The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.

Alternate Names

DKFZp686N0877, SPRY domain containing 4, SPRY domain-containing protein 4

Gene Symbol

SPRYD4

UniProt

NP_997227 (Human)

Additional SPRYD4 Products

Product Documents for SPRYD4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPRYD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.