Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S393-2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPTBN4 Antibodies »

Product Summary for SPTBN4 Antibody (S393-2)

Immunogen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Localization

Cytoplasm , Cytoskeleton , Cytoplasm , Cell Cortex

Specificity

Detects >200kDa. Does not cross react with other Beta-spectrins

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SPTBN4 Antibody (S393-2)

Western Blot: SPTBN4 Antibody (S393-2) [NBP2-59321]

Western Blot: SPTBN4 Antibody (S393-2) [NBP2-59321]

Western Blot: SPTBN4 Antibody (S393-2) [NBP2-59321] - Western Blot analysis of Monkey COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone S393-2 (NBP2-59321). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (NBP2-59321) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
Immunocytochemistry/ Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321]

Immunocytochemistry/ Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321]

Immunocytochemistry/Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SPTBN4 Monoclonal Antibody, Clone S393-2 (NBP2-59321). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SPTBN4 Monoclonal Antibody (NBP2-59321) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SPTBN4 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321]

Immunocytochemistry/ Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321]

Immunocytochemistry/Immunofluorescence: SPTBN4 Antibody (S393-2) [NBP2-59321] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X

Applications for SPTBN4 Antibody (S393-2)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SPTBN4

Alternate Names

beta-IV spectrin, KIAA1642, QV, spectrin beta chain, brain 3, spectrin, beta, non-erythrocytic 4, spectrin, non-erythroid beta chain 3, SPNB4, SPTBN3

Gene Symbol

SPTBN4

Additional SPTBN4 Products

Product Documents for SPTBN4 Antibody (S393-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPTBN4 Antibody (S393-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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