SQLE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32005

Novus Biologicals, part of Bio-Techne
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NBP2-32005
NBP2-32005-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SQLE Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ELHAPLTVVADGLFSKFRKSLVSNKVSVSSHFVGFLMKNAPQFKANHAELILANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMVEKIYPQIPDHLKEPFLEATDNSHLRSMPASFLPPSSVKKRGVLLLGDA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SQLE Antibody

Western Blot: SQLE Antibody [NBP2-32005]

Western Blot: SQLE Antibody [NBP2-32005]

Western Blot: SQLE Antibody [NBP2-32005] - Analysis in human cell line MCF-7 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005]

Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005]

Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005] - Staining in human testis and skeletal muscle tissues using NBP2-32005 antibody. Corresponding SQLE RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005]

Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005]

Immunohistochemistry-Paraffin: SQLE Antibody [NBP2-32005] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
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Applications for SQLE Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SQLE

Squalene epoxidase catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. [provided by RefSeq]

Alternate Names

EC 1.14.99.7, ERG1, FLJ30795, squalene epoxidaseSE, squalene monooxygenase

Gene Symbol

SQLE

UniProt

Q14534 (Human)

Additional SQLE Products

Product Documents for SQLE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SQLE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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