Src Antibody (21H5)

Western Blot: Src Antibody (21H5) [NBP3-26303] -

Western Blot: Src Antibody (21H5) [NBP3-26303] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, MCF-7 whole cell lysate.
All lanes: Src Antibody at 1.2ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 60, 61 KDa
Observed band size: 60, 61 KDa

Immunocytochemistry/Immunofluorescence: Src Antibody (21H5) [NBP3-26303] -

Immunocytochemistry/Immunofluorescence: Src Antibody (21H5) [NBP3-26303] - Staining of MCF-7 cells with Src Antibody (21H5) at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Src Antibody (21H5) [NBP3-26303] -

Flow Cytometry: Src Antibody (21H5) [NBP3-26303] - Overlay histogram showing SH-SY5Y cells stained with Src Antibody (21H5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.