Src [p Ser17] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05433

Novus Biologicals, part of Bio-Techne
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NBP3-05433
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Src Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S17 of human Src (NP_005408.1). RRSLE

Modification

p Ser17

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Src [p Ser17] Antibody - BSA Free

Western Blot: Src [p Ser17] AntibodyBSA Free [NBP3-05433]

Western Blot: Src [p Ser17] AntibodyBSA Free [NBP3-05433]

Western Blot: Src [p Ser17] Antibody [NBP3-05433] - Western blot analysis of extracts of 293T cells, using Src antibody (NBP3-05433) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for Src [p Ser17] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Src

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Long Name

v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog

Alternate Names

ASV, c-Src, RSVgp4

Gene Symbol

SRC

Additional Src Products

Product Documents for Src [p Ser17] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Src [p Ser17] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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