Src [p Tyr527] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13245
![Western Blot: Src [p Tyr527] Antibody [NBP3-13245]](https://resources.bio-techne.com/images/products/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0003.jpg "Western Blot: Src [p Tyr527] Antibody [NBP3-13245]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Chicken (100%), Rhesus Macaque (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human Src (phospho Tyr529). The exact sequence is proprietary. The Tyr529 of human Src is equivalent to Tyr527 of chicken Src.
Modification
p Tyr 527
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Src [p Tyr527] Antibody
![Western Blot: Src [p Tyr527] Antibody [NBP3-13245]](https://resources.bio-techne.com/images/products/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0004.jpg "Western Blot: Src [p Tyr527] Antibody [NBP3-13245]")
Western Blot: Src [p Tyr527] Antibody [NBP3-13245]
Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: Src [p Tyr527] Antibody [NBP3-13245]](https://resources.bio-techne.com/images/products/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0001.jpg "Western Blot: Src [p Tyr527] Antibody [NBP3-13245]")
Western Blot: Src [p Tyr527] Antibody [NBP3-13245]
Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr529) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: Src [p Tyr527] Antibody [NBP3-13245]](https://resources.bio-techne.com/images/products/Src-[p-Tyr527]-Antibody-Western-Blot-NBP3-13245-img0002.jpg "Western Blot: Src [p Tyr527] Antibody [NBP3-13245]")
Western Blot: Src [p Tyr527] Antibody [NBP3-13245]
Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for Src [p Tyr527] Antibody
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Src
Long Name
v-src Sarcoma [Schmidt-Ruppin A-2] Viral Oncogene Homolog
Alternate Names
ASV, c-Src, RSVgp4
Gene Symbol
SRC
Additional Src Products
Product Specific Notices for Src [p Tyr527] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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