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SRD5A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1491

Novus Biologicals, part of Bio-Techne
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NB100-1491

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Guinea Pig

Cited:

Mouse, Guinea Pig

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence ATATGVAEERLLC corresponding to N-Terminus according to NP_001038.1.

Reactivity Notes

Mouse reactivity reported in (PMID: 25797030). Guinea Pig reactivity reported in (PMID: 23585339).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SRD5A1 Antibody

Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]

Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]

Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]

Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]

Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SRD5A1 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4-6 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

1:100 - 1:2000
Application Notes
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Paraffin embedded Human Liver.
WB usage is reported in scientific literature (PMID: 21747118).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-1491 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRD5A1

Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). There are 2 isoforms of the enzyme: SRD5A1 and SRD5A2 (MIM 607306).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 1

Alternate Names

EC 1.3.1.22, S5AR 1, SR type 1

Entrez Gene IDs

6715 (Human)

Gene Symbol

SRD5A1

UniProt

Additional SRD5A1 Products

Product Documents for SRD5A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SRD5A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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