SRD5A1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1491
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Guinea Pig
Cited:
Mouse, Guinea Pig
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence ATATGVAEERLLC corresponding to N-Terminus according to NP_001038.1.
Reactivity Notes
Mouse reactivity reported in (PMID: 25797030). Guinea Pig reactivity reported in (PMID: 23585339).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SRD5A1 Antibody
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491]
Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for SRD5A1 Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
4-6 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
1:100 - 1:2000
Application Notes
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Paraffin embedded Human Liver.
WB usage is reported in scientific literature (PMID: 21747118).
WB usage is reported in scientific literature (PMID: 21747118).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB100-1491 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SRD5A1
Long Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
Alternate Names
EC 1.3.1.22, S5AR 1, SR type 1
Entrez Gene IDs
6715 (Human)
Gene Symbol
SRD5A1
UniProt
Additional SRD5A1 Products
Product Documents for SRD5A1 Antibody
Product Specific Notices for SRD5A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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