SRD5A2 Antibody (8A4H5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15900

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15900-100ul
NBP3-15900-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8A4H5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SRD5A2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRD5A2 (P31213). MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLKPAATRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLLGLFCVHYFHRTFVYSL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRD5A2 Antibody (8A4H5)

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Western Blot: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Analysis of extracts of various cell lines, using SRD5A2 Rabbit mAb (NBP3-15900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Immunofluorescence analysis of mouse testis using SRD5A2 Rabbit mAb (NBP3-15900) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900]

Immunohistochemistry: SRD5A2 Antibody (8A4H5) [NBP3-15900] - Immunofluorescence analysis of rat testis using SRD5A2 Rabbit mAb (NBP3-15900) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for SRD5A2 Antibody (8A4H5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRD5A2

SRD5A2 encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Long Name

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Alternate Names

5 alpha-SR2, S5AR 2, SR type 2

Gene Symbol

SRD5A2

Additional SRD5A2 Products

Product Documents for SRD5A2 Antibody (8A4H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SRD5A2 Antibody (8A4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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