SREBP1 Antibody (SREBP1/1578)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07878
![Immunohistochemistry-Paraffin: SREBP1 Antibody (SREBP1/1578) [NBP3-07878]](https://resources.bio-techne.com/images/products/SREBP1-Antibody-SREBP1-1578-Immunohistochemistry-Paraffin-NBP3-07878-img0001.jpg "Immunohistochemistry-Paraffin: SREBP1 Antibody (SREBP1/1578) [NBP3-07878]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SREBP1/1578
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human SREBP1 protein (Uniprot: P36956)
Localization
Nuclear and Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08432)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for SREBP1 Antibody (SREBP1/1578)
Immunohistochemistry-Paraffin: SREBP1 Antibody (SREBP1/1578) [NBP3-07878]
Immunohistochemistry-Paraffin: SREBP1 Antibody (SREBP1/1578) [NBP3-07878] - IHC analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma. SREBP1 antibody (SREBP1/1578) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.Applications for SREBP1 Antibody (SREBP1/1578)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SREBP1
SREBP1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
Long Name
Sterol regulatory element-binding protein 1
Alternate Names
bHLHd1, SREBF1, SREBP-1
Gene Symbol
SREBF1
UniProt
Additional SREBP1 Products
Product Documents for SREBP1 Antibody (SREBP1/1578)
Product Specific Notices for SREBP1 Antibody (SREBP1/1578)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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