SREBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74542

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-74542
NB100-74542-0.025mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all SREBP1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1.

Localization

Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Nuclear. Multi-pass membrane protein. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SREBP1 Antibody

Western Blot: SREBP1 Antibody [NB100-74542]

Western Blot: SREBP1 Antibody [NB100-74542]

Western Blot: SREBP1 Antibody [NB100-74542] - Analysis of 25 ug of mouse liver (lane 1), rat liver (lane 2) and HepG2 (lane 3) cell lysates onto an SDS polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-74542]

Immunocytochemistry/Immunofluorescence: SREBP1 Antibody [NB100-74542] - Analysis of SREBP1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
View 1 more images

Applications for SREBP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500 - 1:5000
Application Notes
WB: Detects an approx. 68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SREBP1

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Long Name

Sterol regulatory element-binding protein 1

Alternate Names

bHLHd1, SREBF1, SREBP-1

Entrez Gene IDs

20787 (Mouse); 78968 (Rat)

Gene Symbol

SREBF1

UniProt

Q9WTN3 (Mouse)

Additional SREBP1 Products

Product Documents for SREBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SREBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.