SREBP2 Antibody (1D2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54446

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Chicken

Cited:

Human, Mouse, Avian - Chicken

Applications

Validated:

ELISA, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D2

Format

BSA Free

Concentration

1.0 mg/ml

View all SREBP2 Antibodies »

Product Specifications

Immunogen

Human SREBP2 protein (amino acids 48-403). [UniProt# Q12772]

Reactivity Notes

Chicken was reported in PMIDs 15333705 and 11907029.

Localization

Endoplasmic reticulum and Golgi apparatus membranes,cytoplasmic vesicle. Mature/processed form localized to nucleus.

Specificity

Recognizes the precursor and mature forms of human SREBP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SREBP2 Antibody (1D2) - BSA Free

Western Blot: SREBP2 Antibody (1D2)BSA Free [NBP1-54446]

Western Blot: SREBP2 Antibody (1D2)BSA Free [NBP1-54446]

Western Blot: SREBP2 Antibody (1D2) [NBP1-54446] - Lane1: HEp-2 cells. Lane 2: MCF-7 cells. Lane 3: LNCaP cells. Lane 4: HT1080 cells. Lane 5: ZR75-1 cells.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) - BSA Free [NBP1-54446]

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) - BSA Free [NBP1-54446]

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody (1D2) [NBP1-54446] - SREBP2 (1D2) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Applications for SREBP2 Antibody (1D2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunoprecipitation

10 ug/mg protein

Western Blot

2 - 5 ug/mL
Application Notes
In WB, this antibody is expected to detect the precursor as well as the mature forms of SREBP2. Use of this antibody in EMSA/GS (Gel Supershift Assay) and IP applications has been reported in PMIDs 15333705 and 15213220, respectively.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SREBP2

Sterol regulatory element-binding proteins (SREBPs) compose a family of transcriptional factors that regulate the expression of various genes required for the synthesis of phospholipids, fatty acids, and cholesterol. Out of the three isoforms present (SREBP-1a, SREBP-1c and SREBP-2), SREBP-2 has been implicated more directly in the synthesis of cholesterol during metabolism. Similar to SREBP-1a and SREBP-1c, SREBP-2 acts by first binding to sterol regulatory DNA sequences, and subsequently up-regulating the synthesis of enzymes involved in sterol biosynthesis. SREBP-2 is expressed in both the cytoplasm and nucleus on a subcellular level, depending on the state of the protein. It has been known to interact and form a complex with the SCAP protein at the ER, in turn inhibiting interaction between SCAP and SEC24B. This interaction prevents the cleavage of SREBP-2, which results in the synthesis of cholesterol and uptake of LDL despite high levels of present sterols. In recent research, new functions for the SREBP family have offered insight on the effect of lipid metabolism on various pathophysiological diseases (cancers, steatosis and immunity, PMID: 22154484).

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Entrez Gene IDs

6721 (Human)

Gene Symbol

SREBF2

UniProt

Q12772 (Human)

Additional SREBP2 Products

Product Documents for SREBP2 Antibody (1D2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SREBP2 Antibody (1D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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